Cited by W.H. Howell and G.C. Huber, 'A Physiological, Histological and Clinical Study of the Degeneration and Regeneration in Peripheral Nerve Fibres', Journal of Physiology 13 (5) (1892), pp. 335-406
Tags: haemotoxylin, osmic acid, gold
Description:

Explanation of Plate XV (figs. 42-55):

'Fig, 43. To show varicose appearance of newly-formed tube of myeline. Exp, XXVIII. 3/4 in. below wound. Osmic acid and haematoxylin.

Fig. 44. The same. Exp. XXII. R. S. near wrist. Osmic acid and haematoxylin.

Fig. 46. To show the formation of the myeline tube without varicosities. Exp. XXVIII. 3/4 in. below wound. Osmnic acid and haematoxylin.

Fig. 47. To show union of isolated drops of myeline to form a tube. Exp. XXVIII. Osmic acid and haematoxylin.

Fig. 48. To show discontinuous formation of myeline sheath. Exp. XXVIII. Osmic acid and haematoxylin.

Fig. 49, The same. Exp. XXVIII. Osmic acid and haematoxylin.

Fig. 50. To show the persistence of balls of old myeline as yet unabsorbed and their position with reference to the nuclei. Exp. XXVIII. Osmic acid and haematoxylin. Nuclei stained in haematoxylin.

Fig. 51. To show the formation of a node of Ranvier. Exp. XXVIII. Osmic acid and haematoxylin.

Fig. 52. To show the newly-formed myeline tube lying in the embryonic fibre. Exp. IX. 3 weeks. From dorsal cutaneous branch. Gold and haematoxylin.

Fig. 53. The same. Exp. IX. L. S. Dorsal cutaneous branch. Gold and haematoxylin.

Fig. 54. To show the formation of nodes of Ranvier. Exp. IX. L. S. Dorsal cutaneous branch. Gold and haematoxylin.

Fig. 55. To sbow newly-formed myeline tube with node of Ranvier and persistent nuclei. Exp. IX. L. S. 3 weeks. At wound. Gold and haematoxylin.' (404-405)

 

Figs. 42-55 in text:

'Whatever theory of the immediate cause of the formation of the myeline may be the true one, there can be no doubt that it is first found as disconnected drops. These may afterwards become united by slender processes to form a bead-like string which sooner or later elongates to an even tube, or the drops mnay first elongate to form cylindrical segments which eventually unite to form continuous, delicate tubes of myeline. Both of these processes, with the intermediate stages, are shown in Figs. 36 to 55 better than we could describe them in words. What becomes of the numerous nuclei scattered along the embryonic fibre it is not possible to say, other than that they disappear. In some cases, as in Figs. 38, 39, 42, they disappear rapidly as the myeline tube is formed, while in other cases they persist for a much longer time, the newly-formed myeline tube winding in and out among them in a very beautiful manner (see Figs. 37, 51, 55). We can only suppose that they disappear by absorption, as their nutritive relations with reference to the protoplasmic contents of the fibre become less and less important. With reference to the nodes and internodes of Ranvier, it is evident that no simple hypothesis, such as the development of each internode from a single cell, will fit the facts as they appear in regenerating fibres. The developing internodes and nodes are plainly shown in Figs. 51 to 55, but why the ends of the internodal tubes do not fuse together is difficult to explain.' (379-380)

 

Fig. 42 in text:

'the first deposits of myeline, especially when stained in osmic acid, often bear a striking resemblance to the nuclei, both in shape and in the fact that they show a rather distinct staining with the haematoxylin. (See Figs. 38, 39, 40, 42, etc.) There is no doubt that as the myeline forms many of the numerous nuclei scattered along the fibre disappear.' (379)