Cited by T.H. Milroy and J. Malcolm, 'The Metabolism of the Nucleins: Part II. Further observations on excretion in leucocythæmia. Intracellular metabolism in the granular leucocytes', Journal of Physiology 25 (2) (1899), pp. 105-130.
Description:

Explanation of Plate I (figs. 1-6):

'Fig. 1. Marrow Film of Rabbit  I. (control).

Several amphophil cells are shown with well-defined outlines and fairly well-stained nuclei. Two eosinophil myelocytes are seen, the one on the left containing some basophil granules. There are also to be seen an erythioblast with pink areas in its nucleus and a finely granular basophil cell.

Fig. 2. Blood Film of Rabbit I.

Several amphophil cells are shown and one eosinophil in the upper part of the field. The cells are crowded with their granules and the nuclei are distinct.

Fig. 3. Marrow Film of Rabbit Il.

This shows the effect of nucleic acid on the granular cells, eighteen hours after injection. The amphophil cells seen here are larger than normal, with pale, swollen nuclei and granules which have lost their normal oxyphil reaction to a great extent and take on the basic stain. Some granules have been wholly or partially discharged. A large diffuse eosinophil cell is seen with granules altered in reaction.

Fig. 4. Blood Film of Rabbit III.

The nuclei of the amphophil cells are swollen and poor in chromatin. The granules are few and stain in both acid and basic stains. Unstained areas corresponding to granules are also seen. An eosinophil cell with granules altered in reaction is seen to the right.

Fig. 5. Marrow Film of Rabbit III.

Effect fifty hours after last injection. Amphophil cells are seen with very pale nuclei and loosely arranged granules which are altered in reaction. An eosinophil cell which has undergone very marked alterations is seen to the right.

Fig. 6. Marrow Film of Rabbit IV.

Effect seventy-five hours after last injection. Cells recovering. The normal tint of the amphophil granules has to a great extent returned, as is also the case with the eosinophil granules.

Note. The preparations, from which the drawings were made, were fixed by formalin vapour, stained 24 hours in Chenzinsky's eosin-methylene-blue mixture and examined under a 1/12 oil immersion lens, oc. 8 (Zeiss).' (129-130)

 

Fig. 1 in text:

'Rabbit I was uised as a control. The white corpuscles were found to average 5,400 per c.mm., the reds 4,500,000.

Marrow films (Pl. I., Fig. 1). The stains we employed mainly were - Chenzinsky's solution, which was allowed to act for 12 hours at 40° C. in a closed vessel, Ehrlich's triacid mixture, Stohr's eosin and haematoxylin. The colours mentioned later refer in all the experiments to films stained with the eosin and methylene blue mixture.' (117)

 

Fig. 2 int ext:

'Blood (Fig. 2). The amphophil cells were compact and spherical and were filled with fine granules. In some cells the granules appeared to possess slight differences in tint. The coarse oxyphil were very much fewer in number, only about 1-2 % being present, and the numerous granules which they contained were more oxyphil than those of the finely granular cells.' (118)

 

Fig. 3 in text:

'Rabbit II. ...

Marrow films (Fig. 3). Amphophil cells occurred in all parts of the films. They were larger and looser in structure than normal, and there was a tendency for the granules to become scattered.' (119)

 

Fig. 4 in text:

'Blood (Fig. 4). Amphophil cells were larger than in the control, the average increase in diameter being about one-fifth. Their outline was hazy, the nucleus pale and swollen as ,iu the inarrow, and the number of granules per cell was also diminished. In reaction the granules showed marked alterations, some being more oxyphil, others more basophil than normal. In some cases the granules seemed to have entirely disappeared, leaving a homogeneous faint pink groundwork in which unstained areas, corresponding in size to the fine granules, occasionally occurred. Besides these there were amphophil cells present which were apparently unaltered.' (119)

'Blood (Fig. 4). Films were taken from the animal's ear just before death. Here the ordinary polymorphonuclear leucocytes presented a marked contrast to those of the control. The nuclei were pale and swollen and their outlines were less distinct than normal. The cytoplasm extended beyond the distribution of the granules, which were very much less numerous per cell than in the control, the whole structure of the cell being much looser than normal. The granules were in many cases distinctly basophil, though some remained oxyphil. There were very few coarsely granular oxyphil cells present, and those which did remain contained both oxyphil and basophil granules (Fig. 4).' (120-121)

 

Fig. 5 in text:

'Rabbit III. ...

Marrow films (Fig. 5). The granules were diffusely scattered in the cytoplasm of the amphophil cells, which appeared more distended and less compact than the normal finely granular myelocytes. The granules had also changed in reaction, so that now they took on more of the blue tint of those occurring in the basophil cells; but although the majority of the granules had a purely basophil reaction, there were a few present which had not entirely lost the oxyphil tendency, as shown by their violet tint.' (120)

 

Fig. 6 in text:

'Marrow films (Fig. 6). The amphophil cells were very numerous and possessed nuclei which although faintly stained were undoubtedly richer in chromatin than those of rabbit III. The contour of the cells was much the same as that of the control rabbit's myelocytes. The granules were distinctly more oxyphil than those of rabbit III.; a few, however, were still basophil (Fig. 6). The cells as a whole were well preserved, and bearing in mind the appearances seen in the marrow films of the other rabbits, they gave one the impression that they were past the exhaustion stage and were again becoming normal.' (122)