Cited by I.L. Tuckett, 'On the Structure and Degeneration of Non-Medullated Nerve Fibres', Journal of Physiology 19 (4) (1896), pp. 267-311.
Description:

Explanation of Plate IV (figs. 17-21):

'Figures 2, 3a, 3b, 6, 7, 8, 9, 10, 11, 12, 13a, 13b, 14, 17, 18a, 18b, 19, 20, 21a, 21b, 21c, 21d, 21e are drawn with camera lucida and ocular 4, objective V Leitz, magnification 850 diams.

Figures 4a, 4b, 5, 13c, 13d, 15a, 15b, 16a, 16b, 16c, 16d, 16e are drawn with camera lucida and ocular 4, objective 7 Leitz, magnification 590 diams. 

All the figures with the exception of 18 a and 18 b are reduced by 1/3 from the original magnification. The shades of colour in the specimens are represented in the figures as nearly as may be, but it is difficult to get an exact reproduction of the various shades of blue produced by Delafield's heematoxylin and chinolin, Grübler's haematoxylin, methylene blue.' (309)

'Fig. 17. Transverse section of the internal carotid branches of the superior cervical ganglion of a rabbit. Osmic acid 1 per cent. Heematoxylin: (a) nucleus, (b) fibre of Re mak, (c) blood vessel.

Fig. 18 a. Transverse section of the splenic nerve of ox, taken from the centre of the section. Osmic acid .5 per cent. Kupffer's acid fuchsin method: (a) nucleus, (b) varicose part of a fibre, (c) fibre of Remak a little shrunken, (d) granules appearing in core of fibres of Remak.

Fig. 18 b. From the same section but at its edge.

Fig. 19. Bundle of normal fibres of Remak showing a banded appearance. Teased and crushed in aqueous humour. Osmic acid 1 per cent. Gruibler's heematoxylin eosin: (a) nucleus, (b) bundle of fibres.

Fig. 20. Bundle of normal fibres of Remak shewing a vacuolar appearance. Treatment and lettering as above.

Fig. 21 a. Normal fibres of Remak from the right carotid canal of rabbit, No. XVIII. Teased and crushed in aqueous humour. Osmic acid 1 per cent. Grubler's hsematoxylin eosin: (a) nueleus, (b) core of fibre enclosed in sheath.

Fig. 21 b. Two fibres of Remak from the left carotid canal of rabbit, No. XVIII. After 2 days of degeneration. Treatment as above: (a) nucleus, (b) fibre of Remak showing discrete granules.

Fig. 21 c. Two fibres of Remak from the left carotid canal of rabbit, No. II. after 5 days of degeneration. Treatment as above: (a) nucleus, (b) shrunken fibre of Remak.

Fig. 21 d. Two fibres of Remak from the left carotid canal of rabbit, No. XX. After 13 days of degeneration. Treatment and lettering as above.

Fig. 21 e. Two fibres of Remak from the left carotid canal of rabbit, No. XXV. After 231 days of degeneration. Treatment and lettering as above.' (311)

 

Fig. 17 in text:

'Their fineness however constitutes a difficulty: for, owing to it, and the tendency of osmic vapour or osmic acid used sufficiently strong to produce immediate fixation, to cause considerable shrinkage, I have been unable with certainty to obtain transverse sections of normal nerves which showed the outlines of the individual fibres with sufficient clearness. Only occasionally have I been successful (Fig. 17). Therefore I consider that till I am able with certainty always to obtain intelligible transverse sections of these nerves when normal, transverse sections of them when degenerated are worthless. At the same time, as far as I can judge, transverse sections of degenerated nerves of which I have made a considerable number, do not show any striking changes.' (291-292)

 

Fig. 18 in text:

'with reference to the core of the fibres, I have tried Kupffer's acid-fuchsin method several times and I always find that the sheaths of the fibres are stained quite as deeply as the dots which appear in the core of the fibres. But what is still more striking, a great number of the fibres do not show any dots in the centre at all: others show two, three, or even four dots, while others, instead of dots, exhibit a sort of ill-defined granularity (Fig. 18). 

These appearances, in fact, correspond to the granular appearances of fibres of Remak, teased out in iodised serum (Fig. 2). For the core of the fibres is but a process of a nerve-cell, consisting, of rather fluid undifferentiated protoplasm, which is probably granular or, at least, under the influence of osmic acid becomes granular, since nerve-cells after osmic acid hardening are full of granules.' (284)

 

Figs. 19-21 in text:

'With Corrosive Sublimate. In teased preparations the nuclei may be seen to be similar to normal nuclei and the general appearance of the preparation is very similar to normal preparations. But on the whole, I have come to the conclusion that the fibres of Remak seem to consist more than ever of fibrils, and to have a smaller diameter as if something was wanting. Still I do not believe that an observer who had not just been looking at normal non-medullated nerves would be able to distinguish a degenerated from a normal preparation (Fig. 16, a, b, c, d, e).

Osmic Acid. In these preparations it is very necessary to make certain that the fibres under examination are really isolated fibres. For the treatment to which the fibres have been subjected, if it does not isolate the fibres, is very apt to make two or three run together and simulate a very broad fibre of Remak, in which condition various and very curious appearances are sometimes seen (Figs. 19 and 20). In normal fibres, properly isolated, the core is seen to be very finely granular, so as to give an appearance like ground glass. In degenerated nerves after two days the core is seen to be more coarsely granular; which appearance is characteristic of degeneration as long as any core remains. The disappearance of the core varies in different animals: sometimes I have found very little left on the fifth day; sometimes some still remaining on the twelfth day. When the core has disappeared the degenerated preparation can at once be detected by the shrunken state of the fibres; and this is the most obvious sign of degeneration (Figs. 21, a, b, c, d, e). The sheath of the fibres and the nuclei do not seem to be affected by degeneration.' (294)