'One of the chief difficulties attached to the stamping out of glanders in the stable or elsewhere is the difficulty of recognising the disease in its earliest stages. Koch's discovery of tuberculin, and its employment for the diagnosis of tuberculosis, awakened the idea of employing in a similar way, a product of the bacillus of glanders for the diagnosis of glanders and farcy in suspicious cases. In February, 1891, it was reported that in the bacteriological laboratory of the Veterinary Institute at Dorpat an extract had been prepared from the bacillus mallei by means of which the diagnosis of the disease in doubtful cases was assured. This welcome news was, however, shortly followed by the melancholy addendum that Dr. Kalning, the discoverer of the preparation, had contracted the disease himself, and had died.' (701)

'At the Brown Institute mallein kindly furnished by M. Roux, of the Institut Pasteur, As well as some prepared by professor Sherrington at the Institution, has for some time been under trial in London. The results at present obtained have not been altogether so thoroughly satisfactory as those reported from Dantzic; but the number of cases in which trial has been made is still insufficient to afford a basis for positive opinion. (702)