Michaelis-Menten Enzyme Kinetics
Control Panel
About your enzyme
Ref:
{{enzymeParams.code[0]+'/'+enzymeParams.code[1]+enzymeParams.code[2]+'-'+enzymeParams.code[3]+enzymeParams.code[4]+enzymeParams.code[5]+enzymeParams.code[6]+'-'+enzymeParams.code[7]}}
Spec. activity:
{{enzymeParams.sa | number:3}} mmol min-1mg-1
(Est: [S]=20mM; pH=7)
Enzyme conc:
{{enzymeParams.conc | number:3}} mg ml-1
Status
Warning! You are working with an auto-saved enzyme and experiment. To begin a new experiment with a different enzyme, click 'Restart'. Note that you will not be able to return to this enzyme/experiment and results relating to this experiment will be deleted.
Samples:
Working vol:
{{experiment.workingVolume}} µl
Time: {{experiment.timeOfDay | timeFromDecimal}}
Good morning
Setting up took an hour
Lunchtime - take an hour to eat
Tea time - have a break or go home
Working late - getting tired
Working very late - struggling to concentrate
It's time to go home
Hints
Check your reaction params
You need to set up your apparatus - click the 'Setup apparatus' button
You have no defrosted enzyme - click a sample to defrost
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Results
Developed by Dr Damion Young (Medical Sciences Division Learning Technologies), Dr Mark Wormald (Department of Biochemistry) and Dr David Harris (Department of Biochemistry)
Adapted from enzymeLAB v1.2 (Doug Clow, John Garratt and Anne Hodgson, University of York with funding from the Teaching and Learning Technology Programme).
Nonlinear least squares curve fitting developed from original code with kind permission of John C. Pezzullo, PhD: StatPages.info
