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Creators (Definite): Marion Greenwood Bidder; The Cambridge Scientific Instrument CompanyDate: 1886
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Cited by M. Greenwood, 'On the Digestive Process in some Rhizopods. Part II.', Journal of Physiology 8 (5) (1887), pp. 263-310.
Description:Explanation of plate:
'Fig. 9. Actinosphaerium Eichornii. To show reduction of ingesta within their vacuole. Oc. 3, Obj. B.
A 5.30 P.M. a and b are Infusoria.
B 5.35 P.M. At edge of same Actinosphaerium ingestion of c (a small Infusorian) is taking place.
C 6.30 P.M. a, b, c have undergone some reduction.
Fig. 10. Actinosphaerium Eichornii. Oc. 3. Obj. D.
To show large size of excretory vacuole as compared with surrounding structural vacuoles.
d. débris of food (Infusorial) 6 hours after ingestion.
Fig. 11. To indicate some of the various appearances which organisms may present when within Amoeba and Actinosphaerium. A.B.C.D, represent various Infusoria when alive and free.
A. Monas Dallingeri.
B. Scytomonas Pusilla.
D. ? Enchelyodon elongatus.
a, b, c, d represent the same types when they have been with Amoeba (a, b, c) or Actinosphaerium (d) from 30 to 60 minutes.
In all the figures V = digestive vacuole.
In C, are two starch grains (St.) which become more evident in c, from partial solution of the endoplasm in which they lie.
E and F are the probable forerunners of e (Infuisolian) and f (Rotifer) sketched when within the Amoeba: f was ejected 24 hours after the figure was drawn, and during the first 4 hours of this time the vacuole was obvious; it diminished during the next 3 hours and apparently was not formed again.' (286-287)
Fig. 9 in text:
'The act of ingestion. This is sometimes effected without any noteworthy aid from the filiform pseudopodia: these may, it is true, grasp a quiescent prey or cling to an object that is moving, but in the specimens of Actinosphaerium in which I saw most active ingestion, contact of an Infusorian or of Euglena with the main body of its captor was the only antecedent which I could immediately connect with the enclosure that followed. There is a possibility of this enclosure at any point in the circumference of Actinosphaerium; at any point thierefore an ingestive pit may be formed, and films of the surrounding substance may be drawn over the prey. The advance of these films is usually so slow that the steps of it cannot be followed; it may happen however (Pl. IX. fig. 9 B), that when a very active Infusorian is being taken in, a tongue-like protrusion of hyaline substance is seen to run out under it, starting from the middle of the unifornmly present depression.' (279)
Figs. 9 and 10 in text:
'We have to deal then chiefly with bodies that are changed after ingestion, and among them may be distinguished small and larger colourless Infusoria, Rotifers, and small Crustaceans: Euglena is taken up greedily and enclosed Algae may also be found.
Up to a certain point these organisms have a common fate; they are all surrounded by vacuoles, changed while thus surrounded, and ejected from fluid when the digestive act is at an end. (PI. IX. figs. 9 and 10.) As ingestion may take place at any point of the external boundary of the body substance, and as in great activity one ingestion follows another rapidly, it comes to pass that somnetimes many digestive vacuoles, with contents in various stages of change, lie scattered through the body of the Actinosphaerium. And while any vacuole is at first peripheral it may lie more deeply as digestion proceeds, though there is, before loss of food-remains occurs, a final passage towards the superficial layer of the enclosing protoplasm.' (279)
Fig. 10 in text:
'it should be noticed that the fluid of the vacuole as a rtule increases in amount during digestion, and is never so relatively abundant as before the act of ejection. (Pl. IX. fig. 10.)' (280)
'In Actinosphaeriurm it is quite rare to find on any morning traces of organisms which had been ingested on the previous day; and while the "cuticular hooks" of Stylonychia do form a residue which is indigestible, that is, is ejected unchanged, there is quite considerable breaking down of Infusoria in which cuticle and "myophan" layer are well developed. Such Infusoria are generally represented aftex six to eight hours by a colourless granular mass in a large thin walled vacuole (Pl. IX. fig. 10); this mass is speedily discharged and carries with it any accidental food contents of the organism which it represents.' (230-231)