Cited by C.A. MacMunn, 'On the Haematoporphyrin of Solecurtus Strigillatus', Journal of Physiology 8 (6) (1887), pp. 384-390.
Description:

Explanation og Plate XI (figs. 1-4):

'Figs. 1-3. From photomicrographs showing the distribution and morphology of the haematoporphyrin in the epidermis of Solecurtus strigillatus.

Fig. 1. From the foot, magnified 90 diameters.

Fig. 2. From the siphons also magnified 90 diameters.

Fig. 3. Part of the same magnified 280 diameters. The darker parts in all these figures represent the greatest accumulation of pigment. (For full explanation see paper.)

Fig. 4. Spectra of Haematoporphyrin from Solecurtuis strigillatus.

1. Spectrum of a bit of epidermis at base of siphons of Solecurtus strigillatus.

2. Rectified spirit and sulphuric acid extract of the pigment, shewing bands of Acid haematoporphyrin.

3. The pigment was isolated by chloroform as described in the paper, the solution evaporated and the residue dissolved in rectified spirit to which a little ammonia was added; the spectrum is that of a mixture of acid and alkaline haematoporphyrin.

4. To the solution mentioned more ammonia was added, when the spectrum of 3 changed to this; that of alkaline haematoporphyrin. The very narrow and faint band before D is not constant in solutions of haematoporphyrin.' (389-390)

 

Fig. 1 in text:

'Solecurtus strigillatus is a mollusk which belongs to the family Solenidae...

On looking at such a specimen one is at once struck with the peculiar colour which is brown and the whole surface of the animal is more or less coloured except where it is attached to the shell...

On examination with the microscope the pigment is seen to be distributed - in the foot especially - in the manner shown in Pl. XI. fig. 1 from a photomicrograph, which shews that the bulk of the pigment occurs in the lines forming the mesh-work; these appear to mark the boundary between the epithelial cells or islands and this appearance recalls to mind the so-called endothelium cells stained with nitrate of silver.' (384)

 

Figs. 2-3 in text:

'In the siphons the distribution of pigment is different, the edge of each ring being deeply pigmented so that the space enclosed between successive rings appears dark; the approximation of the rings not being complete leaves however a narrow bright channel between the rings as shown in fig. 2. The edges of the rings are wavy and under a quarterinch objective are serrated, while the rings themselves are channelled more or less incompletely as shewn in figs. 2 and 3; in fig. 3 however being slightly out of focus in order to shew the granuilar appearance of the pigment between them.' (385)

 

Fig. 4 in text:

'The Spectroscopic Characters of the Pigment. Pl. XI. Fig. 4. The pigment is limited entirely to the surface of the animal and if a portion deeply enough coloured to give distinct bands be mounted in balsam after dehydrating in the usual manner, examined with the microspectroscope and compared with a similarly mounted specimen of Moseley's polyperythrin [note: 'Quart. Journz. Micros. Soc. Vol. xvii. 1887, pp. 1-23.'], e.g. from Flabellum, the bands are found to be identical both as regards position and relative shading. They are also identical with those in a similarly prepared specimnen from the dorsal streak of Lumbricus terrestris. If the coloured portions be extracted with alcohol acidulated with sulphuric acid and the alcohol then filtered a purple red solution is obtained which gives the acid haematoporphyrin spectrum well marked, P1. XI. Fig. 4 (sp. 2).' (385-386)

 

Figs. 1-3. From photomicrographs showing the distribution and morphology of the haematoporphyrin in the epidermis of Solecurtus strigillatus. Fig. 1. From the foot, magnified 90 diameters. Fig. 2. From the siphons also magnified 90 diameters. Fig. 3. Part of the same magnified 280 diameters. The darker parts in all these figures represent the greatest accumulation of pigment. (For full explanation see paper.)