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Creators (Definite): F. Huth; John Newport Langley
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Cited by J.N. Langley, 'On the Changes in Serous Glands during Secretion', Journal of Physiology 2 (4) (1879), pp. 261-322.
Description:Explanation of Plate VII:
'Parotid gland of the rabbit in the fresh state. The figures are all from the same gland. In drawing the outlines a camera was used, Zeiss microscope, oc. 2, obj. E.
Fig. 1. Small piece of fresh gland taken at 12 o'clock. The clear lines marking the junctions of the cells are made a little too broad.
Fig. 2. Taken at 1.45, after 3.65 c.c. of saliva had been obtained by injecting pilocarpin in small quantities into the duct.
Fig. 3. Taken at 3.30, after 1 c.c. saliva more from pilocarpin; and following stimulation of sympathetic in neck for 10" to 15" every 60" during 55'.
Fig. 4. Taken at 5.16, after stimulating sympathetic as above for 100'; altogether 1.6 c.c. saliva from sympathetic stimulation.
Fig. 5. Resting gland after 10' mounting in saliva.
Fig. 6. Resting gland after 20' mounting in saliva.' (279) [nb: see comments re: delineation in description of Plate VIII]
Fig. 1 in text:
'The alveoli of the parotid of a hungry rabbit taken fresh from the animal and at once examined, are granular throughout (Pl. VII. Fig. 1); there is no division into an outer clear and an inner granular zone; the cells of the alveoli can be made out with more or less distinctness as clear lines running between the granules.' (263)
Figs. 2-4 in text:
'As the granules disappear from a cell the rest of the granules do not form a small collection at the part bordering the lumen, but form rather lines at the margin and sides near the lumen (cp. Figs. 2, 3 and 4). The cells too, as has been pointed out by Heidenhain [note: 'Pflüger's Archiv. Bd. xvii. S. 1, 1878.'], become smaller.' (364)
Fig. 4 in text:
'The longer the gland is stimulated the more obvious the outer clear zone becomes, so that at length some alveoli may shew scarcely any granules. (Fig. 4.) At this stage the secretion offered by the gland is very scanty.' (263-264)
Figs. 5-6 in text:
'I have attempted to reproduce the appearance offered by the gland as the cells become acted on by the fluid. Fig. 5 (Pl. VII.) is from a resting gland ten minutes after being mounted in saliva. Fig. 6 from a resting gland twenty minutes after being mounted in saliva. The clear border appearing at a certain stage of the action of the fluid on the alveoli is totally different in appearance from the "non-granular" zone appearing in the alveoli on stimulation. The nuclei become visible only after the fluid has acted on them for some time. The distinction of granular and non-granular zones disappears or becomes very obscure on the addition of either alcohol, chromic acid, picric acid, or osmic aceid.' (264-265)