'The mosquitoes are given an opportunity of biting by applying the gauze-covered end of the feeding jar to the external surface of the patient’s thigh (vide supra). If the patient is residing a distance of several hours’ journey from the laboratory, two batches of insects are often taken, and these are given an opportunity of feeding immediately on arrival, and again a few hours later. To be absolutely certain that the transmission will be effected, an attempt is made to get at least 10 mosquitoes to bite, but with the heavily infected insects commonly used, a much smaller number would be sufficient. [note: ‘On some occasions when the insects have shown little inclination to bite, the infected salivary glands have been dissected out, crushed, and suspended in Locke’s fluid, as described by James, Nicol and Shute (1927). The resultant suspension was then injected intravenously. This procedure is also very useful for experimental purposes, and makes it possible to inoculate a known number of sporozoites (Shute, 1937).’]' (19)